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ATCC
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Image Search Results
Journal:
Article Title: Maintenance of caspase-3 proenzyme dormancy by an intrinsic "safety catch" regulatory tripeptide
doi: 10.1073/pnas.111085198
Figure Lengend Snippet: Acidification disables the safety catch and promotes procaspase-3 activation. (a) Ac-DEVD-AMC cleavage activity in procaspase-3 expressing bacterial extracts after incubation in buffers at varying pH levels. (b) α-Caspase-3 immunoblot and corresponding Ac-DEVD-AMC cleavage activity in cytosolic extracts from the neuronal precursor cells NT2 treated as described in a.
Article Snippet: Lysates were prepared from the E. coli strain BL21(DE3) pLysS as described above or from the human
Techniques: Activation Assay, Activity Assay, Expressing, Incubation, Western Blot